Overview

Basics

Basics
Using microcosm laboratory experiments that mimicked winter and summer-like conditions under the rubber barriers, this project examined: -Whether fecal indicator bacteria such as total coliforms, fecal coliforms, Escherichia coli and enterococci re-grow under the barrier. -Whether artificially added human pathogens (Campylobacter jejuni and Salmonella enterica) re-grow and/or persist. -Whether alternative fecal indicator bacteria such as universal-, human-, dog- and bovine-associated Bacteroidales re-grow. -The amount of ammonium, phosphate and dissolved organic carbon released under the barrier as a result of decaying Asian clams.

Completed
2009
2010
2012
$104,237

Kat McIntyre (KMcIntyre@trpa.gov)
10/27/2017
10/27/2017
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Location Information Location Notes

Organizations

EIP Details

EIP Basics

04.01.01.0095 - P056: Potential for Pathogen Growth, Fecal Indicator Growth and Phosphorus Release under Clam Removal Barriers in the LTB

Performance Measures

Expected Performance Measures

Expected Performance Measures

No expected performance measures set for this project.

Reported Performance Measures

Reported Performance Measures

No annual performance measure accomplishments entered for this project.

Funding

Expected Funding

Expected Funding
$104,237
$104,237
$0

Total
Southern Nevada Public Land Management Act (Pacific Southwest Research Station) (USFS - PSW) $104,237 $104,237 $0
Grand Total $104,237 $104,237 $0

Reported Expenditures

Reported Expenditures
Total 2012 2011 2010 2009
Southern Nevada Public Land Management Act (Pacifi... $104,237 $26,060 $26,059 $26,059 $26,059
Grand Total $104,237 $26,060 $26,059 $26,059 $26,059

Photos

Photos

No photos available.

Other Details

Watersheds

No watersheds set for this project.

Threshold Categories

  • Asian clams (Corbicula fluminea) are considered a major threat to the ecosystem in Lake Tahoe; since their discovery in April 2008 they have increased in density to visibly impair the near-shore areas both through the production of shells and through facilitating the growth of nuisance periphyton (attached algae) and metaphyton (filamentous, non-attached algae).

Local and Regional Plans

No Local and Regional Plans set for this project.

Related Projects

Related Projects

No Related Projects set for this project.

External Links

No external links entered.

Notes

Notes
08/14/2017 2:15 PM Kiara Cuerpo-Hadsall Objectives:

The overall objective of this study was to evaluate impacts of the rubber barrier installation on microbial and chemical water quality. Specific questions addressed here were:

• Whether fecal indicator bacteria (FIB) such as total coliforms, fecal coliforms, Escherichia coli and enterococci re-grow under the barrier.

• Whether artificially added human pathogens (Campylobacter jejuni and Salmonella enterica) re-grow and/or persist.

• Whether alternative fecal indicator bacteria such as universal-, human,- dog- and bovine-associated Bacteroidales re-grow.

• How much nutrients (ammonium, phosphate and dissolved organic carbon (DOC) would be released under the barrier as a result of decaying Asian clams.

08/14/2017 2:14 PM Kiara Cuerpo-Hadsall Findings:

• The study aimed to evaluate impacts of rubber barrier installations on water quality in Lake Tahoe. A microcosm study was performed, mimicking environmental conditions under the rubber barriers in the laboratory. We found the following results:

• FIB (fecal indicator bacteria) did not increase under the rubber barriers at winter temperatures in any of the cases studies, whereas sporadic increases in FIB, especially total coliforms, were observed in some cases at summer temperatures.

• The model pathogens Campylobacter jejuni and Salmonella enterica did not significantly increase in numbers under the barriers at either winter or summer temperatures as measured by DNA. The pathogen decay rate constants at summer temperatures, however, were lower than those reported under ambient water conditions elsewhere, indicating that these pathogens persisted longer under rubber barriers.

• Host-associated Bacteroidales DNA did not increase at either winter or summer temperatures, whereas universal-Bacteroidales DNA showed a slight increase at summer temperatures.

• Dissolved Organic Carbon (DOC) release rates were the highest followed by ammonium and phosphate at both winter and summer temperatures. Nutrient release rates at summer temperatures were one order of magnitude higher than at winter temperatures. Release rates of ammonium and phosphate estimated at summer temperatures were 10 to 1000 times higher than release rates from sediment reported in Lake Tahoe, suggesting that dead Asian clams were possible sources.

08/14/2017 2:13 PM Kiara Cuerpo-Hadsall Management Implications:

In conclusion, considering the fact that no FIB increase was observed and lower nutrient release rates were measured at winter-like conditions, installation of rubber barriers during winter could minimize the impacts on water quality, but this could also lead to a longer lead time before achieving 100% Asian clam mortality.

08/14/2017 2:12 PM Kiara Cuerpo-Hadsall Publications: www.fs.fed.us/PSW/partnerships/tahoescience

Mitsunori Odagiri, Alexander Schriewer, Geoff Schladow, and Stefan Wuertz, Potential for Pathogen Growth, Fecal Indicator Growth and Phosphorus Release under Clam Removal Barriers in the Lake Tahoe Basin. Final Report (P 056), June 2012.